O b j e c t i v e s: To evaluate the properties of natural sweetener
solutions in whole organ preservation and assess their influence on the
dimension, weight and shape of cardiac tissue samples in stated time
intervals, up to a one-year period of observation.
B a c k g r o u n d: Tissue fixation is essential for biological sample
examination. Many negative toxic effects of formaldehyde-based fixatives
have forced us to seek alternatives for formaldehyde based solutions. It
has been demonstrated that natural sweeteners can preserve small tissue
samples well and that these solutions can be used in histopathological
processes. However, their ability to preserve whole human organs are
M e t h o d s: A total of 30 swine hearts were investigated. Th ree
study groups (n = 10 in each case) were formed and classifi ed on the
type of fixative: (1) 10% formaldehyde phosphate-buffered solution
(FPBS), (2) 10% alcohol-based honey solution (ABHS), (3) 10% water-based
honey solution (WBHS). Samples were measured before fi xation and in the
following time points: 24 hours, 72 hours, 168 hours, 3 months, 6 months
and 12 months.
R e s u l t s: The WBHS failed to preserve heart samples and
decomposition of tissues was observed one week after fixation. In half
of the studied parameters, the ABHS had similar modifying tendencies as
compared to FPBS. Th e overall condition of preserved tissue, weight,
left ventricular wall thickness, right ventricular wall thickness and
the diameter of the papillary muscle differed considerably.
C o n c l u s i o n s: The ABHS may be used as an alternative fi xative
for macroscopic studies of cardiac tissue, whereas the WBHS is not
suited for tissue preservation.