The reliable and rapid diagnosis of infectious animal diseases presents an exceptionally im- portant aspect when considering their control and prevention. The paper describes the compara- tive evaluation of two rapid isothermal amplification methods for diagnosis of African swine fever (ASF). The robustness of loop-mediated isothermal amplification (LAMP) and the cross-priming amplification (CPA) were compared using samples obtained from ASF confirmed animals. Both assays were evaluated in order to define their diagnostic capabilities in terms of ASF diagnosis and reproducibility of the results. Investigations showed no cross-reactivity for other pig patho- gens and no significant differences in the specificity of both assays. The sensitivity of LAMP reached 90%, while that of CPA was 70%. In conclusion, both methods are suitable for imple- mentation in preliminary ASF diagnosis but further improvements are required to enhance their diagnostic sensitivity.
The paper provides statistical analysis of the photographs of four various granular materials (peas, pellets, triticale, wood chips). For analysis, the (parametric) ANOVA and the (nonparametric) Kruskal-Wallis tests were applied. Additionally, the (parametric) two-sample t-test and (non-parametric) Wilcoxon Rank-Sum Test for pairwise comparisons were performed. In each case, the Bonferroni correction was used. The analysis shows a statistical evidence of the presence of differences between the respective average discrete pixel intensity distributions (PID), induced by the histograms in each group of photos, which cannot be explained only by the existing differences among single granules of different materials. The proposed approach may contribute to the development of a fast inspection method for comparison and discrimination of granular materials differing from the reference material, in the production process.
The article refers to the idea of using the software defined network (SDN) as an effective hardware and software platform enabling the creation and dynamic management of distributed ICT infrastructure supporting the rapid prototyping process. The authors proposed a new layered reference model remote distributed rapid prototyping that allows the development of heterogeneous, open systems of rapid prototyping in a distributed environment. Next, the implementation of this model was presented in which the functioning of the bottom layers of the model is based on the SDN architecture. Laboratory tests were carried out for this implementation which allowed to verify the proposed model in the real environment, as well as determine its potential and possibilities for further development. Thus, the approach described in the paper may contribute to the development and improvement of the efficiency of rapid prototyping processes which individual components are located in remote industrial, research and development units. Thanks to this, it will be possible to better integrate production processes as well as optimize the costs associated with prototyping. The proposed solution is also a response in this regard to the needs of industry 4.0 in the area of creating scalable, controllable and reliable platforms.
Four commercial disinfectants were chosen for being generally accepted as effective against ASFV. Only two of them, based on sodium hypochlorite and potassium peroxymonosulfate, confirmed their effectiveness in selected concentrations. Taken together, our data supports the effectivenes of chemical disinfectants containing sodium hypochlorite (1%, 0.5% in low level soiling) and potassium peroxymonosulfate (1% in high level soiling). Furthermore, these results highlight the importance of pre-cleaning steps to remove soiling before proper disinfection which improves the effectiveness of tested disinfectants.