The objective of this study was to determine the applicability of the Migratest® kit for evaluating the chemotactic activity of peripheral blood neutrophils in goats. The experiment was performed on 14 goat kids aged 30±2 days, divided into two groups of 7 animals each: C - control group, and E – experimental group, supplemented with β-hydroxy-β-methylbutyrate (HMB), a typical immunostimulant which influences the phagocytic activity of peripheral neutrophils. The feed administered to experimental goat kids was supplemented with HMB at 40 mg/kg BW, whereas control goat kids were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the chemotactic activity of peripheral blood neutrophils in goats. The results of the study indicate that the Migratest® kit can be used to evaluate the influence of immunomodulators on the chemotactic activity of peripheral blood neutrophils in goats. The results of the assay are most effectively presented by calculating the chemotactic index which accounts for the chemotaxis or migration of neutrophils in the presence or absence of a chemotactic factor, respectively, and the percentage of granulocytes that migrate towards fMLP. The results of both presentation methods appear to be identical.
The aim of the present study was to investigate whether the anterior chamber constitutes part of the normal migratory pathway of CD4+ and CD8+ lymphocytes in cattle and swine. The cells obtained from aqueous humor of cows and pigs were stained for CD4 and CD8 receptors, and subsequently analyzed with flow cytometry. The mean percentage of CD4+CD8-, CD4-CD8+ and CD4+CD8+ cells within the total lymphocyte population of the bovine anterior chamber was, respectively, 17.88, 12.64 and 27.26%. In turn, the mean values of these parameters in pigs were 1.77, 38.48 and 17.45, respectively. Among bovine and porcine CD4+CD8+ cells prevalent were those displaying CD4lowCD8low and CD4lowCD8high phenotypes, respectively. The results suggest that the anterior chamber in cattle and swine is an element in the normal migratory pathway of CD4+, CD8+ and CD4+CD8+ cells. Furthermore, the contribution of these subsets in the anterior chamber lymphocyte population can differ considerably between animal species.