Influence of the initial grain size on hot deformation behavior of the low-alloy Mn-Ti-B steel was investigated. The uniaxial compression tests were performed in range of the deformation temperatures of 900-1200°C and strain rates of 0.1-10 s–1. One set of samples was heated directly to the deformation temperature, which corresponded to the initial austenitic grain size of 19-56 μm; the other set of samples was uniformly preheated at the temperature of 1200°C. Whereas the values of activation energy, peak stress and steady-state stress values practically did not depend on the initial austenitic grain size, the peak strain values of coarser-grained structure significantly increase mainly at high values of the Zener-Hollomon parameter. This confirms the negative effect of the large size of the initial grain on the dynamic recrystallization kinetics, which can be explained by the reduction in nucleation density.